(S)-Binaphthol derivative-bonded phases were prepared for direct chiral separation by high-performance liquid chromatography. The bonded phases were prepared by methylation of the hydroxyl groups or introduction of aryl groups at 3,3'-positions in the binaphthol moiety. Methylation varied retention and enantioselectivity for amines, which clarified that the hydroxyl groups are essential for chiral recognition of amines. Substitution of phenyl or naphthyl groups at 3,3'-positions of the binaphthol moiety increased both hydrophobicity and steric hindrance, which also vary retention and enantioselectivity of analytes. Copyright (C) 1998 Elsevier Science B.V.