Increased luminescence of the glosensor cAMP assay in lβt2 cells does not correlate with cAMP accumulation under low PH conditions

Shiori Musha, Syo Murakami, Ryotaro Kojima, Hideaki Tomura

研究成果: Article

1 被引用数 (Scopus)

抄録

Cyclic adenosine monophosphate (cAMP) plays a pivotal role in gonadotrope responses in the pituitary. Gonadotropin-releasing hormone (GnRH) mediated synthesis and secretion of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) are regulated by both the Gs/cAMP and Gq/Ca2+ signaling pathways. Pituitary adenylate cyclaseactivating polypeptide (PACAP) also regulates GnRH responsiveness in gonadotropes through the PACAP receptor, which activates the Gs/cAMP signaling pathway. Therefore, measuring intracellular cAMP levels is important for elucidating the molecular mechanisms of FSH and LH synthesis and secretion in gonadotropes. The GloSensor cAMP assay is useful for detecting cAMP levels in intact, living cells. In this study, we found that increased GloSensor luminescence intensity did not correlate with cAMP accumulation in LβT2 cells under low pH conditions. This result indicates that cell type and condition must be considered when using GloSensor cAMP.

本文言語English
ページ(範囲)381-388
ページ数8
ジャーナルJournal of Reproduction and Development
65
4
DOI
出版ステータスPublished - 2019

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