Genomic SELEX screening of regulatory targets of Escherichia coli transcription factors

Tomohiro Shimada, Hiroshi Ogasawara, Akira Ishihama

研究成果: Chapter査読

16 被引用数 (Scopus)

抄録

The genome of Escherichia coli K-12 is transcribed by a single species of RNA polymerase. The selectivity of its transcriptional targets is modulated via two-steps of protein-protein interaction: at the first step, seven species of the sigma subunit are involved, at the second step, a total of approximately 300 species of transcription factor (TFs). For the identification of the regulatory targets of these two groups of regulatory proteins, we developed two in vitro approaches, “Genomic SELEX” (currently designated as gSELEX) and “PS (promoter-specific)-TF” screenings. Here, we describe a detailed protocol of the genomic SELEX screening system which uses purified regulatory proteins and fragments of genomic DNA from E. coli.

本文言語English
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ49-69
ページ数21
DOI
出版ステータスPublished - 2018

出版物シリーズ

名前Methods in Molecular Biology
1837
ISSN(印刷版)1064-3745

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