Determination of monosaccharides derivatized with 2-aminobenzoic acid by capillary electrophoresis

Mitsuru Abo, Li Ping He, Kae Sato, Akira Okubo

研究成果: Chapter査読

1 被引用数 (Scopus)

抄録

Reducing monosaccharides were derivatized with 2-aminobenzoic acid (2-AA) through reductive amination using sodium cyanoborohydride as a reductant, and the derivatives were separated by capillary zone electrophoresis with UV detection using 50 mM sodium phosphate (pH 5.5) or 150 mM sodium borate - 50 mM sodium phosphate (pH 7.0) running buffer. The derivatives of monosaccharides, which are major components of various carbohydrate materials, were completely separated within 25 min.

本文言語English
ホスト出版物のタイトルCapillary Electrophoresis of Biomolecules
ホスト出版物のサブタイトルMethods and Protocols
出版社Humana Press Inc.
ページ45-50
ページ数6
ISBN(印刷版)9781627032957
DOI
出版ステータスPublished - 2013

出版物シリーズ

名前Methods in Molecular Biology
984
ISSN(印刷版)1064-3745

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