Biocompatibility of silver-Containing calcium-Phosphate cements with anti-Bacterial properties

Yusuke Shimizu, Yusuke Kawanobe, Toshiisa Konishi, Nobuyuki Kanzawa, Michiyo Honda, Mamoru Aizawa

研究成果: Conference contribution


We have previously synthesized silver-containing hydroxyapatite (Ag-HAp) powders by an ultrasonic spray-pyrolysis (USSP) technique. On the other hand, we have successfully fabricated novel calcium-phosphate cements (CPCs) composed of mainly |3-tricalcium phosphate (|3-TCP) phase with anti-washout property (hereafter, |3-TCP cement), which was set on the basis of chelate-bonding ability of inositol phosphate (IP6). In this study, we developed novel CPCs with both anti-bacterial and anti-washout properties by adding the Ag-HAp powder into the above |3-TCP cements, and examined their anti-bacterial property and cytotoxicity. The Ag-HAp powders with Ag contents of 0, 2, and 5 mol% as a nominal composition were synthesized by an USSP technique. The raw powder for β-TCP cement was prepared by ball-milling the commercially-available |3-TCP powder in the IP6 solution. The Ag-HAp/β-TCP powders were prepared by mixing Ag-HAp powder and β-TCP cement powder at a ratio of 25:75 in mass. The Ag-HAp/|3-TCP cement was fabricated by mixing the above-mentioned Ag-HAp/β-TCP powder and 2.5 mass% Na2HP04solution at a powder/liquid ratio of 1/0.3 [g/cm3 ]. The anti-bacterial property of resulting cements was evaluated using Staphylococcus aureus by biofilm formation test. The Ag-HAp/β-TCP cements containing 2 and 5 mol% Ag showed strong anti-bacterial property among examined specimens. Furthermore, the cytotoxicity of Ag+ ion eluted from these cements was also examined using osteoblastic MC3T3-E1 cells and Transwell® kit. The relative cell viability cultured on each Ag-containing cement specimen was over 80 %, compared with the control (polystyrene plate). These results demonstrate that the present Ag-HAp/β-TCP cements containing 2 mol% Ag are promising one of the candidates as CPCs with both anti-bacterial property and biocompatibility.

ホスト出版物のタイトルBioceramics 26
編集者Maria-Pau Ginebra, Cristina Canal, Montserrat Espanol, Edgar B. Montufar, Roman A. Perez
出版者Trans Tech Publications Ltd
出版物ステータスPublished - 1 1 2015
イベント26th Symposium and Annual Meeting of the International Society for Ceramics in Medicine, ISCM 2014 - Barcelona, Spain
継続期間: 6 11 20148 11 2014


名前Key Engineering Materials


Conference26th Symposium and Annual Meeting of the International Society for Ceramics in Medicine, ISCM 2014


Shimizu, Y., Kawanobe, Y., Konishi, T., Kanzawa, N., Honda, M., & Aizawa, M. (2015). Biocompatibility of silver-Containing calcium-Phosphate cements with anti-Bacterial properties. : M-P. Ginebra, C. Canal, M. Espanol, E. B. Montufar, & R. A. Perez (版), Bioceramics 26 (pp. 107-112). (Key Engineering Materials; 巻数 631). Trans Tech Publications Ltd.