@inbook{fef787f7e04a483a89f1c37497c6a6fa,
title = "Affinity labeling and purification of plant chitin-binding LysM receptor with chitin octasaccharide derivatives",
abstract = "Lysin motif (LysM) is a carbohydrate-binding modules found in all kingdoms. LysM binds to N-acetylglucosamine-containing molecules such as peptidoglycan, chitin, Nod factor, and Myc factor and is found in peptidoglycan hydrolases, chitinases, and plant pathogen effectors and plant receptor/co-receptor for defense and symbiosis signaling. This chapter describes the synthesis of a nonradioactive chitin ligand, biotinylated chitin octasaccharide, (GlcNAc)8-Bio, and its application for the detection and characterization of chitin-binding LysM receptor CEBiP in the microsomal membrane fraction of rice suspension-cultured cells by affinity labeling. We also describe the purification of CEBiP from the plasma membrane of the rice cells by affinity chromatography with the synthesized (GlcNAc)8-APEA-CH-Sepharose as an affinity matrix.",
keywords = "Affinity labeling, Affinity ligand, Biotinylation, CEBiP, Chitin oligosaccharide, Chitin receptor, LysM, Purification",
author = "Tomonori Shinya and Naoto Shibuya and Hanae Kaku",
year = "2020",
doi = "10.1007/978-1-0716-0430-4_39",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "401--412",
booktitle = "Methods in Molecular Biology",
}