Affinity labeling and purification of plant chitin-binding LysM receptor with chitin octasaccharide derivatives

Tomonori Shinya, Naoto Shibuya, Hanae Kaku

研究成果: Chapter査読

抄録

Lysin motif (LysM) is a carbohydrate-binding modules found in all kingdoms. LysM binds to N-acetylglucosamine-containing molecules such as peptidoglycan, chitin, Nod factor, and Myc factor and is found in peptidoglycan hydrolases, chitinases, and plant pathogen effectors and plant receptor/co-receptor for defense and symbiosis signaling. This chapter describes the synthesis of a nonradioactive chitin ligand, biotinylated chitin octasaccharide, (GlcNAc)8-Bio, and its application for the detection and characterization of chitin-binding LysM receptor CEBiP in the microsomal membrane fraction of rice suspension-cultured cells by affinity labeling. We also describe the purification of CEBiP from the plasma membrane of the rice cells by affinity chromatography with the synthesized (GlcNAc)8-APEA-CH-Sepharose as an affinity matrix.

本文言語English
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ401-412
ページ数12
DOI
出版ステータスPublished - 2020

出版物シリーズ

名前Methods in Molecular Biology
2132
ISSN(印刷版)1064-3745
ISSN(電子版)1940-6029

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