Fusarium graminearum species complex produces type B trichothecenes oxygenated at C‐ 7. In axenic liquid culture, F. graminearum mainly accumulates one of the three types of trichothe-cenes, namely 3‐acetyldeoxyinvalenol, 15‐acetyldeoxyinvalenol, or mixtures of 4,15‐diacetylniva-lenol/4‐acetylnivalenol, depending on each strain’s genetic background. The acetyl groups of these trichothecenes are slowly deacetylated to give deoxynivalenol (DON) or nivalenol (NIV) on solid medium culture. Due to the evolution of F. graminearum FgTri1, encoding a cytochrome P450 monooxygenase responsible for hydroxylation at both C‐7 and C‐8, new derivatives of DON, des-ignated as NX‐type trichothecenes, have recently emerged. To assess the risks of emergence of new NX‐type trichothecenes, we examined the effects of replacing FgTri1 in the three chemotypes with FgTri1_NX chemotype, which encodes a cytochrome P450 monooxygenase that can only hydroxylate C‐7 of trichothecenes. Similar to the transgenic DON chemotypes, the transgenic NIV chemotype strain accumulated NX‐type 4‐deoxytrichothecenes in axenic liquid culture. C‐4 oxygenated trichothe-cenes were marginal, despite the presence of a functional FgTri13 encoding a C‐4 hydroxylase. At present, outcrossing of the currently occurring NX chemotype with NIV chemotype strains of F. graminearum in the natural environment likely will not yield a new strain that produces a C‐4 oxygenated NX‐type trichothecene.