Transcription factor-promoter interaction networks

Akira Ishihama, Tomohiro Shimada, Hiroshi Ogasawara, Jun Teramoto, Kaneyoshi Yamamoto

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The pattern of genome transcription in Escherichia coli determined by controlling the distribution of a total of about 2,000 molecules of RNA polymerase, the transcriptase, among 4,000 genes on its genome. In response to environmental changes, the gene selectivity of the RNA polymerase is modulated after interaction with one sigma subunit, the promoter recognition subunit, in the first step and one or two of about 300 transcription factors in the second step. In order to get insights into the molecular mechanisms underlying the global regulation of genome expression, we have initiated a systematic study of the identification of genes under the control of each transcription factor using the newly developed 'Genomic SELEX' system. The gene regulation predicted by the Genomic SELEX is being examined by analysis of the promoter activity in the presence and absence of test transcription factors using the newly constructed two-fluorescent-protein promoter assay vector pGRP. Results altogether indicate the involvement of complex networks between transcription factors and promoters in the global regulation of genome transcription.

Original languageEnglish
Title of host publicationProceedings of the 2005 International Symposium on Micro-NanoMechatronics and Human Science, Eighth Symposium on Micro- and Nano-Mechatronics for Information-Based Society - The 21st Century COE Progr
Pages153-157
Number of pages5
DOIs
Publication statusPublished - 1 Dec 2005
Event2005 International Symposium on Micro-NanoMechatronics and Human Science, Eighth Symposium on Micro- and Nano-Mechatronics for Information-Based Society - The 21st Century COE Program - Nagayo, Japan
Duration: 7 Nov 20059 Nov 2005

Publication series

NameProceedings of the 2005 International Symposium on Micro-NanoMechatronics and Human Science, Eighth Symposium on Micro- and Nano-Mechatronics for Information-Based Society - The 21st Century COE Progr
Volume2005

Conference

Conference2005 International Symposium on Micro-NanoMechatronics and Human Science, Eighth Symposium on Micro- and Nano-Mechatronics for Information-Based Society - The 21st Century COE Program
Country/TerritoryJapan
CityNagayo
Period7/11/059/11/05

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