Sweat Lactic Acid Biosensor using Continuous Flow as Sample Career

Yoshiki Tojyo, Haruna Saito, Keigo Enomoto, Hiroyuki Kudo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

A microfluidic biosensor for real-time monitoring of lactic acid (LA) contained in sweat was fabricated and tested. Sweat is one of the most promising body fluids for non-invasive assessment of biological components reflecting physiological condition. In order to measure sweat LA continuously, we developed a microfluidic biosensor, which is highly specific with LA, and a sampling element that transports whole secretions to the biosensor. In this study, the biosensor was first fabricated and its fundamental characteristics were investigated. The biosensor showed sufficient sensitivity (0.16 nA μM -1 ) to measure sweat LA and high specific activity suitable for the sweat monitoring purpose was also confirmed. Real-sample test was also carried out using our system. The microfluidic biosensor was attached on the upper arm of healthy male subject. Sweat LA was continuously monitored during bike exercise. As a result, elevation of LA was successfully detected (62.4 μg/cm 2 /min for anaerobic exercise), and the signal decreased to the background level after the exercise. This possibility of non-invasive evaluation of metabolic activity was demonstrated.

Original languageEnglish
Title of host publicationBMEiCON 2018 - 11th Biomedical Engineering International Conference
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781538657249
DOIs
Publication statusPublished - 10 Jan 2019
Event11th Biomedical Engineering International Conference, BMEiCON 2018 - Chaing Mai, Thailand
Duration: 21 Nov 201824 Nov 2018

Publication series

NameBMEiCON 2018 - 11th Biomedical Engineering International Conference

Conference

Conference11th Biomedical Engineering International Conference, BMEiCON 2018
CountryThailand
CityChaing Mai
Period21/11/1824/11/18

Keywords

  • Biomonitoring
  • Biosensor
  • Electrochemical
  • Lactic acid
  • Real-time

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  • Cite this

    Tojyo, Y., Saito, H., Enomoto, K., & Kudo, H. (2019). Sweat Lactic Acid Biosensor using Continuous Flow as Sample Career. In BMEiCON 2018 - 11th Biomedical Engineering International Conference [8609931] (BMEiCON 2018 - 11th Biomedical Engineering International Conference). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/BMEiCON.2018.8609931