Single molecule analysis of transcription factor-DNA complexes using atomic force microscopy

Masahiro Nakano, Jun Teramoto, Tomohiro Shimada, Kaneyoshi Yamamoto, Akira Ishihama

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Transcription of the Escherichia coli genome is under the control of approximately 300 species of transcription factor. However, the regulatory roles remain unidentified for about 100 species of the transcription factor. As an approach to identify their regulation modes, we analyzed the dynamics of DNA-transcription factor interactions using the atomic force microscopy (AFM). Here we examined the DNA-binding mode of two types of transcription factor in the presence and absence of RNA polymerase: a novel growth-specific nucleoid protein Dan (DNA-binding protein under anaerobic growth conditions); and global regulators, CRP (cAMP receptor protein) and Cra (catabolite repressor and activator), for control of transport and metabolism of carbon sources.

Original languageEnglish
Title of host publication2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation"
PublisherIEEE Computer Society
Pages490-494
Number of pages5
ISBN (Print)9781457713613
DOIs
Publication statusPublished - 1 Jan 2011
Event22nd Annual Symp. on Micro-Nano Mechatronics and Human Science, MHS 2011, Held Jointly with the Symp. on COE for Education and Research of Micro-Nano Mechatronics, Micro-Nano GCOE 2011, Symp. on Hyper Bio Assembler for 3D Cellular System Innovation - Nagoya, Japan
Duration: 6 Nov 20119 Nov 2011

Publication series

Name2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation"

Conference

Conference22nd Annual Symp. on Micro-Nano Mechatronics and Human Science, MHS 2011, Held Jointly with the Symp. on COE for Education and Research of Micro-Nano Mechatronics, Micro-Nano GCOE 2011, Symp. on Hyper Bio Assembler for 3D Cellular System Innovation
Country/TerritoryJapan
CityNagoya
Period6/11/119/11/11

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