Selective differentiation of bone marrow-derived mesenchymal stromal cells into osteocytes via endochondral ossification in an apatite-fiber scaffold

Michiyo Honda, Yuka Watanabe, Takahide Tsuchiya, Nobuyuki Kanzawa, Mamoru Aizawa

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3 Citations (Scopus)


Bone marrow-derived mesenchymal stromal cells (BMSCs) are pluripotent progenitor cells that can regenerate different skeletal tissues in response to environmental signals. Scaffolds play a critical role in tissue engineering, and their microstructure is essential for inductive bone formation. In the present study, we have used highly porous, structurally stable three-dimensional apatite-fiber scaffolds (AFSs) and investigated their ability to support cell proliferation and differentiation. BMSCs in/on AFSs were proliferated in a three-dimensional manner when both micro- and macro-pores were present. The characteristic structure of the AFS enhanced calcification, production of extracellular matrix (ECM), and osteogenic differentiation, especially endochondral ossification. Furthermore, BMSCs cultured in/on AFSs did not undergo adipogenesis and selectively underwent osteogenic differentiation. These results suggest that AFSs provide a suitable environment for bone regeneration and have potential applications for tissue engineering.

Original languageEnglish
Pages (from-to)759-765
Number of pages7
JournalNippon Seramikkusu Kyokai Gakujutsu Ronbunshi/Journal of the Ceramic Society of Japan
Issue number1417
Publication statusPublished - 1 Sep 2013


  • Bone marrow-derived stromal cell
  • Differentiation
  • Endochondral ossification
  • Hydroxyapatite
  • Scaffold

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