Physiological roles of semenogelin I and zinc in sperm motility and semen coagulation on ejaculation in humans

Kaoru Yoshida, Natsuko Kawano, Miki Yoshiike, Manabu Yoshida, Teruaki Iwamoto, Masaaki Morisawa

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

At ejaculation, human sperm are considered to be mechanically trapped and become immotile in the semen coagulum by binding to semenogelins (Sgs) from the seminal vesicle and zinc ions from the prostate. However, the physiological combined roles of the protein and heavy metal on sperm motility are unknown. Here, we have first demonstrated that Sg I alone, which does not form the semen coagulum without zinc, is an inhibitor of the motility of intact human sperm at physiological concentration. On the other hand, zinc ions alone had no effect on sperm motility, but confer recovery of sperm motility that has been inhibited by Sg I at a concentration equal to or less than 1 mg/ml. These observations suggest that the roles played by Sg I and zinc on sperm motility are not mechanical but physiological. Quartz crystal microbalance analysis suggests that the sperm extract first bind to Sg I and then zinc ions which subsequently increase the protein accumulation, suggesting that Sgs inhibit sperm motility by directly binding to the sperm surface. Further accumulation of Sg I mediated by zinc ions may entrap the quiescent sperm at semen ejaculation.

Original languageEnglish
Pages (from-to)151-156
Number of pages6
JournalMolecular Human Reproduction
Volume14
Issue number3
DOIs
Publication statusPublished - 1 Mar 2008

Keywords

  • Human sperm
  • Motility
  • Semenogelin I
  • Zinc

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