Micro-CT of small insects by projection X-ray microscopy

Akira Tanisako, Susumu Tsuruta, Ayumi Hori, Aya Okumura, Chikara Miyata, Chiaki Kuzuryu, Takashi Obi, Hideyuki Yoshimura

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)


The projection X-ray microscope utilized a very small X-ray source emitted from a thin (0.1-3 μm) target metal film excited by the focused electron beam of a scanning electron microscope (SEM). When an object is placed just below the target metal film, the diverging X-rays enlarge the shadow of the object. Because no X-ray optics such as a zone-plate is used, the focal depth is, in principle, infinitely large. We exploited this to apply projection X-ray microscopy to three-dimensional (3-D) structure analysis by means of cone-beam computed tomography (CT). A small arthropod (Pseudocneorhinus bifasciatus, 5 mm in length) was examined for CT study. The projection images were recorded at 3-degree increments over the whole range (360°) of a stepping-motor- controlled sample rotator. The 3-D reconstructed image was calculated to be 256 × 256 × 256 (5 μm) voxel data. The reconstructed 3-D image showed in detail the internal structure of an opaque object. Trial for element mapping using projection X-ray microscope is also performed by developing a new target exchanger. This apparatus enables exchange of metal targets without leaking vacuum of SEM. By taking images using Ka line from nickel and cobalt targets, distribution of iron, which has absorption edge between two Ka lines, can be shown. Distribution of less than 10 μm iron particles is distinguished from cobalt particles. This system would be applicable for 3-D element analysis.

Original languageEnglish
Title of host publicationDevelopments in X-Ray Tomography V
Publication statusPublished - 13 Nov 2006
EventDevelopments in X-Ray Tomography V - San Diego, CA, United States
Duration: 15 Aug 200617 Aug 2006

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceDevelopments in X-Ray Tomography V
CountryUnited States
CitySan Diego, CA



  • Computer tomography
  • Element mapping
  • Internal structure
  • Micro CT
  • Projection X-ray microscope

Cite this

Tanisako, A., Tsuruta, S., Hori, A., Okumura, A., Miyata, C., Kuzuryu, C., ... Yoshimura, H. (2006). Micro-CT of small insects by projection X-ray microscopy. In Developments in X-Ray Tomography V [63180B] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6318). https://doi.org/10.1117/12.679780