L-Theanine elicits umami taste via the T1R1 + T1R3 umami taste receptor

Masataka Narukawa, Yasuka Toda, Tomoya Nakagita, Yukako Hayashi, Takumi Misaka

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)


L-Theanine is a unique amino acid present in green tea. It elicits umami taste and has a considerable effect on tea taste and quality. We investigated L-theanine activity on the T1R1 + T1R3 umami taste receptor. L-Theanine activated T1R1 + T1R3-expressing cells and showed a synergistic response with inosine 5′-monophosphate. The site-directed mutagenesis analysis revealed that L-theanine binds to L-amino acid binding site in the Venus flytrap domain of T1R1. This study shows that L-theanine elicits an umami taste via T1R1 + T1R3.

Original languageEnglish
Pages (from-to)1583-1587
Number of pages5
JournalAmino Acids
Issue number6
Publication statusPublished - Jun 2014


  • L-Theanine
  • Synergy
  • Umami


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