Isolation and culture conditions of a klebsiella pneumoniae strain that can utilize ammonium and nitrate ions simultaneously with controlled iron and molybdate ion concentrations

Young Ju Kim, Makoto Yoshizawa, Shinji Takenaka, Shuichiro Murakami, Kenji Aoki

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

A bacterial strain F-5-2, isolated from soil and identified as Klebsiella pneumoniae, removed NH4+ completely in 24 h of aerobic cultivation in a medium containing 1 mg/ml of NH4NO3. However, 70% of the NO3 originally provided remained. When 100 μM Fe2+ was added to the medium, both NH4+ and NO3 were removed simultaneously and completely from the culture within 6 h of incubation. In addition, the amount of MoO4 in the medium markedly affected the bacterial cell growth and utilization of NH4+ and NO3. The bacterium could remove 4 mg/ml of NH4NO3 completely in 48 h of aerobic cultivation in a medium containing 100 μM Fe2+ and 0.8 pM MoO42−. The total nitrogen in the culture containing its cells was decreased to 14% of that in the NH4NO3 originally provided. GC-MS analysis demonstrated that N2 was generated from the nitrogen atoms of both NH4+ and NO3.

Original languageEnglish
Pages (from-to)996-1001
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume66
Issue number5
DOIs
Publication statusPublished - 2002

Keywords

  • Aerobic denitrification
  • Ammonium nitrate
  • Heterotrophic nitrification
  • Iron ion
  • Molybdate ion

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