In vitro evaluation of chelate-setting cements fabricated from silicon-containing apatite powder using osteoblastic cells

Yusuke Nakashima, Michiyo Honda, Toshiisa Konishi, Minori Mizumoto, Mamoru Aizawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In our previous study, silicon-containing hydroxyapatite (Si-HAp) powder was prepared via an aqueous precipitation reaction. The Si-HAp powders were synthesized with desired Si contents (0, 0.4, 0.8, 1.6, and 2.4 mass%) as a nominal composition. Another previous study in our group demonstrated surface-modification of HAp powder with inositol phosphate (IP6) enhanced the compressive strength of apatite cement. Thus, to fabricate the cements with higher bioactivity, the above Si-HAp powders were surface-modified with IP6 (IP6-Si-HAp). The IP6-Si-HAp cements with various Si contents were fabricated by mixing with pure water at the powder/liquid ratio of 1/0.4 [w/v]. In order to clarify biocompatibility of the IP6-Si-HAP cements in the present work, MC3T3-E1 cells as a model of osteoblast were seeded on the cement specimens. As for the numbers of cells cultured on the IP6-Si-HAp cements, the substitution of lower levels of Si into HAp lattice did not greatly influence the cell proliferation. However, the substitution of Si amount over 0.8 mass% enhanced the cell proliferation. Especially, the IP6-Si-HAp cement with the Si content of 2.4 mass% showed excellent cell proliferation among examined specimens. Therefore, to fabricate the cements with higher bioactivity, it is necessary to control the amount of Si in the IP6-Si-HAp cements. The usage of these IP6-Si-HAp cements may make it possible to fabricate the cements with higher bioactivity, compare to conventional pure HAp cements.

Original languageEnglish
Title of host publicationBioceramics 24
Pages183-186
Number of pages4
Edition1
DOIs
Publication statusPublished - 1 Jan 2013
Event24th Symposium and Annual Meeting of International Society for Ceramics in Medicine, ISCM 2012 - Fukuoka, Japan
Duration: 21 Oct 201224 Oct 2012

Publication series

NameKey Engineering Materials
Number1
Volume529-530
ISSN (Print)1013-9826

Conference

Conference24th Symposium and Annual Meeting of International Society for Ceramics in Medicine, ISCM 2012
CountryJapan
CityFukuoka
Period21/10/1224/10/12

Keywords

  • Apatite
  • Cement
  • Osteoblast
  • Silicon
  • Silicon-containing hydroxyapatite

Cite this

Nakashima, Y., Honda, M., Konishi, T., Mizumoto, M., & Aizawa, M. (2013). In vitro evaluation of chelate-setting cements fabricated from silicon-containing apatite powder using osteoblastic cells. In Bioceramics 24 (1 ed., pp. 183-186). (Key Engineering Materials; Vol. 529-530, No. 1). https://doi.org/10.4028/www.scientific.net/KEM.529-530.183