Hybrid lethality in interspecific hybrids between Nicotiana tabacum and N. suaveolens: Evidence that the Q chromosome causes hybrid lethality based on Q-chromosome-specific DNA markers

T. Tezuka, Wataru Marubashi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Hybrid seedlings from the cross Nicotiana tabacum x N. suaveolens express lethality at 28°C. We carried out a cross between monosomic lines of N. tabacum lacking the Q chromosome and N. suaveolens by test-tube pollination and ovule culture at 28°C. To suppress hybrid lethality, hybrid seedlings obtained were transferred to 36°C immediately after germination and cultured. We determined whether Q-chromosome-specific DNA markers were detected among hybrid seedlings. When hybrid seedlings cultured at 36°C were transferred to 28°C, hybrid seedlings in which Q-chromosome-specific DNA markers were detected expressed hybrid lethality, while hybrid seedlings in which Q-chromosome-specific DNA markers were not detected did not express hybrid lethality. From these results, we concluded that the presence of the Q chromosome of N. tabacum is related to hybrid lethality observed in crosses between N. tabacum and N. suaveolens. This is the first report that clearly demonstrates the relationship between a certain chromosome and hybrid lethality in the genus Nicotiana using chromosome-specific DNA markers. Additionally, we confirmed that the Q chromosome belongs to the S subgenome because Q-chromosome-specific DNA markers were detected only in N. sylvestris. S

Original languageEnglish
Pages (from-to)1172-1178
Number of pages7
JournalTheoretical and Applied Genetics
Volume112
Issue number6
DOIs
Publication statusPublished - 1 Apr 2006

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