Heat treatment temporarily suppresses expression of programmed cell death in hybrid tobacco cells (Nicotiana suaveolens x N. tabacum) expressing hybrid lethality

Satoshi Kobori, Yu Masuda, Wataru Marubashi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Features of programmed cell death (PCD), including nuclear fragmentation and DNA ladders were detected in hybrid cells of Nicotiana suaveolens X N. tabacum expressing hybrid lethality at 28°C, but not in cells kept at 36°C. Heat treatment (HT, 50°C for 15 min) before transfer to 28°C from 36°C temporarily suppressed the increase in the percentage of dead cells. In hybrid cells without HT, the percentage of Sub G1 nuclei, corresponding to those with nuclear fragmentation increased at 6 h after transfer to 28°C and DNA ladders were detected at 9 h after transfer to 28°C. On the other hand, in hybrid cells with HT, the percentage of Sub G1 increased and DNA ladders were detected 15 h after transfer to 28°C. These results suggest that HT temporarily suppresses PCD during expression of hybrid lethality.

Original languageEnglish
Pages (from-to)345-348
Number of pages4
JournalPlant Biotechnology
Volume22
Issue number4
DOIs
Publication statusPublished - Dec 2005

Keywords

  • Heat treatment
  • Hybrid lethality
  • Nicotiana
  • Programmed cell death

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