Generation of a felinized swine endothelial cell line by expression of feline decay-accelerating factor

Luna Izuhara, Norifumi Tatsumi, Shuji Miyagawa, Satomi Iwai, Masahito Watanabe, Shuichiro Yamanaka, Yuichi Katsuoka, Hiroshi Nagashima, Hirotaka J. Okano, Takashi Yokoo

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Abstract

Embryonic stem cell research has facilitated the generation of many cell types for the production of tissues and organs for both humans and companion animals. Because ≤30% of pet cats suffer from chronic kidney disease (CKD), xenotransplantation between pigs and cats has been studied. For a successful pig to cat xenotransplant, the immune reaction must be overcome, especially hyperacute rejection. In this study, we isolated the gene for feline decay-accelerating factor (fDAF), an inhibitor of complement proteins, and transfected a swine endothelial cell line with fDAF to "felinize" the pig cells. These fDAF-expressing cells were resistant to feline serum containing anti-pig antibodies, suggesting that felinized pig cells were resistant to hyperacute rejection. Our results suggest that a "felinized" pig kidney can be generated for the treatment of CKD in cats in the future.

Original languageEnglish
Article numbere0117682
JournalPLoS ONE
Volume10
Issue number2
DOIs
Publication statusPublished - 11 Feb 2015

Cite this

Izuhara, L., Tatsumi, N., Miyagawa, S., Iwai, S., Watanabe, M., Yamanaka, S., Katsuoka, Y., Nagashima, H., Okano, H. J., & Yokoo, T. (2015). Generation of a felinized swine endothelial cell line by expression of feline decay-accelerating factor. PLoS ONE, 10(2), [e0117682]. https://doi.org/10.1371/journal.pone.0117682