Fabrication of gentamicin-loaded hydroxyapatite/collagen bone-like nanocomposite for anti-infection bone void fillers

Sho Oshima, Taira Sato, Michiyo Honda, Yasushi Suetsugu, Kazuhide Ozeki, Masanori Kikuchi

Research output: Contribution to journalArticle


A gentamicin-loaded hydroxyapatite/collagen bone-like nanocomposite (GNT-HAp/Col) was fabricated and evaluated for its absorption–desorption properties, antibacterial efficacy, and cytotoxicity. The hydroxyapatite/collagen bone-like nanocomposite (HAp/Col) powder was mixed with gentamicin sulfate (GNT) in phosphate-buffered saline (PBS) at room temperature. After 6 h mixing, the GNT adsorption in all conditions reached plateau by Langmuir’s isotherm, and maximum GNT adsorption amount was 34 ± 7 µg in 250 µg/mL GNT solution. Saturated GNT-loaded HAp/Col powder of 100 mg was soaked in 10 mL of PBS at 37 C and released all GNT in 3 days. A shaking culture method for a GNT extraction from the GNT-HAp/Col and an inhibition zone assay for the GNT-HAp/Col compact showed antibacterial efficacy to Escherichia coli (E. coli) at least for 2 days. From the release profile of the GNT from the GNT-HAp/Col powder, antibacterial efficacy would affect E. coli at least for 3 days. Further, no cytotoxicities were observed on MG-63 cells. Thus, the GNT-HAp/Col is a good candidate of bioresorbable anti-infection bone void fillers by prevention initial infections, which is the primary cause of implant-associated infection even for rapid bioresorbable materials.

Original languageEnglish
Article number551
JournalInternational Journal of Molecular Sciences
Issue number2
Publication statusPublished - 2 Jan 2020



  • Antibacterial activity
  • Antibiotic-impregnated biomaterial
  • Bone void filler
  • Gentamicin sulfate
  • Hydroxyapatite/collagen bone-like nanocomposite

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