Efficient extraction and preservation of thermotolerant phycocyanins from red alga Cyanidioschyzon merolae

Chihiro Yoshida, Miyabi Murakami, Anna Niwa, Masahiro Takeya, Takashi Osanai

Research output: Contribution to journalArticlepeer-review

Abstract

C-Phycocyanin (PC) is a protein used commercially as a natural blue pigment produced by cyanobacteria, cryptophytes, and rhodophytes. Although it is industrially synthesized from the cyanobacterium Arthrospira platensis, PC requires high levels of energy for its extraction, which involves freezing of cells. However, as a protein, PC is easily denatured at extreme temperatures. In this study, we extracted PC from the red alga Cyanidioschyzon merolae, denoted as CmPC, and found that this protein was tolerant to high temperatures and acidic pH. CmPC was extracted by suspending cells in water mixed with various salts and organic acids without freeze-drying or freeze-thaw. The stability of CmPC varied with salt concentration and was destabilized by organic acids. Our results indicate that C. merolae is a potential candidate for PC production with thermotolerant properties.

Original languageEnglish
Pages (from-to)161-167
Number of pages7
JournalJournal of Bioscience and Bioengineering
Volume131
Issue number2
DOIs
Publication statusPublished - Feb 2021

Keywords

  • Cyanidioschyzon merolae
  • Microalgae
  • Phycocyanin
  • Protein extraction
  • Red algae

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