Effect of paternal genome excess on the developmental and gene expression profiles of polyspermic zygotes in rice

Ryouya Deushi, Erika Toda, Shizuka Koshimizu, Kentaro Yano, Takashi Okamoto

Research output: Contribution to journalArticlepeer-review

Abstract

Polyploid zygotes with a paternal gamete/genome excess exhibit arrested development, whereas polyploid zygotes with a maternal excess develop normally. These observations indicate that paternal and maternal genomes synergistically influence zygote development via distinct func-tions. In this study, to clarify how paternal genome excess affects zygotic development, the developmental and gene expression profiles of polyspermic rice zygotes were analyzed. The results indicated that polyspermic zygotes were mostly arrested at the one-cell stage after karyogamy had com-pleted. Through comparison of transcriptomes between polyspermic zygotes and diploid zygotes, 36 and 43 genes with up-regulated and down-regulated expression levels, respectively, were iden-tified in the polyspermic zygotes relative to the corresponding expression in the diploid zygotes. Notably, OsASGR-BBML1, which encodes an AP2 transcription factor possibly involved in initiating rice zygote development, was expressed at a much lower level in the polyspermic zygotes than in the diploid zygotes.

Original languageEnglish
Article number255
Pages (from-to)1-13
Number of pages13
JournalPlants
Volume10
Issue number2
DOIs
Publication statusPublished - Feb 2021

Keywords

  • Fertilization
  • Male excess
  • Parental genome
  • Paternal genome
  • Polyspermy
  • Rice

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