A giant surface potential (GSP) has been observed on a tris-(8- hydroxyquinolate) aluminum (Alq3) film deposited on a glass or a metal substrate under dark conditions. However, the effects of a GSP on the device properties of Alq3-based organic light-emitting diodes (OLEDs) has not been considered. In this paper, we report on the effects of ambient light during the fabrication of an Alq3-based OLED on the device properties by displacement current measurement. We found that the light irradiation significantly reduces the density of charge existing at the 4,4′-bis[N-(1-naphthyl)-N-phenylamino]-biphenyl/Alq3 interface and results in the formation of charge traps in the Alq3 layer. Considering the similarities between the GSP and the interfacial charge, they can be attributed to the same origin; the orientaion polarization of Alq 3 film.