Differentiation of THP-1 monocytes to macrophages increased mitochondrial DNA copy number but did not increase expression of mitochondrial respiratory proteins or mitochondrial transcription factor A

Mizuho Okamoto, Masanori Shimogishi, Akari Nakamura, Yusuke Suga, Kyosuke Sugawara, Michio Sato, Ryotaro Nishi, Akio Fujisawa, Yorihiro Yamamoto, Misato Kashiba

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Monocytes are differentiated into macrophages. In this study, mitochondrial DNA copy number (mtDNAcn) levels and downstream events such as the expression of respiratory chain mRNAs were investigated during the phorbol 12-myristate 13-acetate (PMA)-induced differentiation of monocytes. Although PMA treatment increased mtDNAcn, the expression levels of mRNAs encoded in mtDNA were decreased. The levels of mitochondrial transcription factor A mRNA and protein were also decreased. The levels of coenzyme Q10 remained unchanged. These results imply that, although mtDNAcn is considered as a health marker, the levels of mtDNAcn may not always be consistent with the parameters of mitochondrial functions.

Original languageEnglish
Article number108988
JournalArchives of Biochemistry and Biophysics
Volume710
DOIs
Publication statusPublished - 15 Oct 2021

Keywords

  • Coenzyme Q10
  • Macrophage
  • mtDNA copy number
  • PMA
  • TFAM
  • THP-1

Fingerprint

Dive into the research topics of 'Differentiation of THP-1 monocytes to macrophages increased mitochondrial DNA copy number but did not increase expression of mitochondrial respiratory proteins or mitochondrial transcription factor A'. Together they form a unique fingerprint.

Cite this