Development of an Electrochemical Immunosensor for Salivary Oxytocin Determination

Airi Asano, Mako Shiibashi, Hiroyuki Kudo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A miniaturized electrochemical-oxytocin (OXT) sensor using competitive ELISA was constructed and tested. The OXT sensor measures OXT as reduction of glucose oxidase-labeled oxytocin, which competitively captured by anti-OXT antibodies. We employed a nitrocellulose membrane as a support for antibody because the nitrocellulose membrane improved the output signal by two folds comparing with using a microtiter plate as a support. In the experiment, competitive immunoreaction was first optically measured using conventional biotinylated capture antibody and avidin-GOD. As a result, it was confirmed that oxytocin measurement was possible by the constructed immune system. After that, oxytocin was electrochemically measured on the electrode using a carbon graphite electrode. Redox currents showed a relationship with oxytocin concentration and the calibration range involved 15pg/mL, which has been reported as salivary oxytocin.

Original languageEnglish
Title of host publicationBMEiCON 2018 - 11th Biomedical Engineering International Conference
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781538657249
DOIs
Publication statusPublished - 10 Jan 2019
Event11th Biomedical Engineering International Conference, BMEiCON 2018 - Chaing Mai, Thailand
Duration: 21 Nov 201824 Nov 2018

Publication series

NameBMEiCON 2018 - 11th Biomedical Engineering International Conference

Conference

Conference11th Biomedical Engineering International Conference, BMEiCON 2018
CountryThailand
CityChaing Mai
Period21/11/1824/11/18

Keywords

  • Competitive ELISA
  • Electrochemical
  • Immunosensor I
  • Oxytocin

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  • Cite this

    Asano, A., Shiibashi, M., & Kudo, H. (2019). Development of an Electrochemical Immunosensor for Salivary Oxytocin Determination. In BMEiCON 2018 - 11th Biomedical Engineering International Conference [8609952] (BMEiCON 2018 - 11th Biomedical Engineering International Conference). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/BMEiCON.2018.8609952