Determination of monosaccharides derivatized with 2-aminobenzoic acid by capillary electrophoresis

Mitsuru Abo, Li Ping He, Kae Sato, Akira Okubo

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Citation (Scopus)

Abstract

Reducing monosaccharides were derivatized with 2-aminobenzoic acid (2-AA) through reductive amination using sodium cyanoborohydride as a reductant, and the derivatives were separated by capillary zone electrophoresis with UV detection using 50 mM sodium phosphate (pH 5.5) or 150 mM sodium borate - 50 mM sodium phosphate (pH 7.0) running buffer. The derivatives of monosaccharides, which are major components of various carbohydrate materials, were completely separated within 25 min.

Original languageEnglish
Title of host publicationCapillary Electrophoresis of Biomolecules
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages45-50
Number of pages6
ISBN (Print)9781627032957
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
Volume984
ISSN (Print)1064-3745

Keywords

  • 2-Aminobenzoic acid
  • Capillary zone electrophoresis
  • Reductive amination

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