Control with organic solvents of efficiency of persolvent cholesterol fermentation by pseudomonas sp. Strain ST-200

Noriyuki Doukyu, Hideki Kobayashi, Harushi Nakajima, Rikizo Aono

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Pseudomonas sp. strain ST-200 oxidizes cholesterol dissolved in an organic solvent overlying the medium. Major conversion products are cholest-4-en-3-one (C4EO), 6β-hydroxycholest-4-en-3-one (HCEO), and cholest-4-ene-3,6-dione (CEDO). Productivity of each conversion product was altered by changing organic solvents used to dissolve the cholesterol. Generally, HCEO was predominant among the products. HCEO was produced even by cells grown without cholesterol and then killed with harmful organic solvents. The yield of the most oxidized product, CEDO, was improved when the cells were grown in the presence of cholesterol dissolved in a less toxic solvent, cyclooctane.

Original languageEnglish
Pages (from-to)1612-1616
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Issue number10
Publication statusPublished - 1 Jan 1996


  • Bioconversion of cholesterol
  • Cholesterol conversion
  • Organic solvents
  • Persolvent fermentation
  • Pseudomonas sp

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