Collective nuclear behavior shapes bilateral nuclear symmetry for subsequent left-right asymmetric morphogenesis in Drosophila

Dongsun Shin, Mitsutoshi Nakamura, Yoshitaka Morishita, Mototsugu Eiraku, Tomoko Yamakawa, Takeshi Sasamura, Masakazu Akiyama, Mikiko Inaki, Kenji Matsuno

Research output: Contribution to journalArticlepeer-review

Abstract

Proper organ development often requires nuclei to move to a specific position within the cell. To determine how nuclear positioning affects left-right (LR) development in the Drosophila anteriormidgut (AMG), we developed a surface-modeling method to measure and describe nuclear behavior at stages 13-14, captured in three-dimensional timelapse movies. We describe the distinctive positioning and a novel collective nuclear behavior by which nuclei align LR symmetrically along the anterior-posterior axis in the visceral muscles that overlie the midgut and are responsible for the LR-asymmetric development of this organ. Wnt4 signaling is crucial for the collective behavior and proper positioning of the nuclei, as aremyosin II and the LINCcomplex, without which the nuclei fail to align LR symmetrically. The LR-symmetric positioning of the nuclei is important for the subsequent LR-asymmetric development of the AMG. We propose that the bilaterally symmetrical positioning of these nuclei may be mechanically coupled with subsequent LR-asymmetric morphogenesis.

Original languageEnglish
Article number198507
JournalDevelopment (Cambridge)
Volume148
Issue number18
DOIs
Publication statusPublished - Sep 2021

Keywords

  • 3D reconstruction
  • Actin
  • Development
  • Image processing
  • Nucleus

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