Cell cycle arrest and the induction of apoptosis in pancreatic cancer cells exposed to adenosine triphosphate in vitro

Takayuki Yamada, Fumikazu Okajima, Mohammed Akbar, Hideaki Tomura, Torao Narita, Tatsuya Yamada, Susumu Ohwada, Yasuo Morishita, Yoichi Kondo

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14 Citations (Scopus)

Abstract

Adenosine triphosphate (ATP) has been shown to be an inhibitory or a stimulatory agent for cell growth in various types of cells. Here, we studied the effects of extracellular ATP on two pancreatic cancer cell lines, PK-1 and YAPC established by us. In both cell lines, ATP inhibited cell growth in a time- and dose-dependent manner, whereas the same doses of ATP stimulated DNA synthesis. Flow cytometric analysis of the cells incubated with or without ATP demonstrated the ATP-induced striking increase in cells at S-phase. The same analysis showed also the increase in sub-G0/G1 population in the same analysis and the electrophoretic pattern of DNA showed the occurrence of ATP-induced cell disintegration likely to be apoptosis. We suggest that extracellular ATP is cytotoxic for pancreatic cancer cells because of its induction of cell cycle arrest at S-phase and cell death, possibly apoptosis, overcoming the promotion of the entry into S-phase.

Original languageEnglish
Pages (from-to)113-117
Number of pages5
JournalOncology Reports
Volume9
Issue number1
Publication statusPublished - 1 Jan 2002

Keywords

  • Adenosine triphosphate
  • Apoptosis
  • Cell cycle
  • Cell proliferation
  • Pancreatic cancer

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    Yamada, T., Okajima, F., Akbar, M., Tomura, H., Narita, T., Yamada, T., Ohwada, S., Morishita, Y., & Kondo, Y. (2002). Cell cycle arrest and the induction of apoptosis in pancreatic cancer cells exposed to adenosine triphosphate in vitro. Oncology Reports, 9(1), 113-117.