C-terminal regulatory domain of the ε subunit of FoF1 ATP synthase enhances the ATP-dependent H+ pumping that is involved in the maintenance of cellular membrane potential in Bacillus subtilis

Genki Akanuma, Tomoaki Tagana, Maho Sawada, Shota Suzuki, Tomohiro Shimada, Kan Tanaka, Fujio Kawamura, Yasuyuki Kato-Yamada

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

The ε subunit of FoF1-ATPase/synthase (FoF1) plays a crucial role in regulating FoF1activity. To understand the physiological significance of the ε subunit-mediated regulation of FoF1in Bacillus subtilis, we constructed and characterized a mutant harboring a deletion in the C-terminal regulatory domain of the ε subunit (ε∆C). Analyses using inverted membrane vesicles revealed that the ε∆C mutation decreased ATPase activity and the ATP-dependent H+-pumping activity of FoF1. To enhance the effects of ε∆C mutation, this mutation was introduced into a ∆rrn8 strain harboring only two of the 10 rrn (rRNA) operons (∆rrn8 ε∆C mutant strain). Interestingly, growth of the ∆rrn8 ε∆C mutant stalled at late-exponential phase. During the stalled growth phase, the membrane potential of the ∆rrn8 ε∆C mutant cells was significantly reduced, which led to a decrease in the cellular level of 70S ribosomes. The growth stalling was suppressed by adding glucose into the culture medium. Our findings suggest that the C-terminal region of the ε subunit is important for alleviating the temporal reduction in the membrane potential, by enhancing the ATP-dependent H+-pumping activity of FoF1.

Original languageEnglish
Article numbere00815
JournalMicrobiologyOpen
Volume8
Issue number8
DOIs
Publication statusPublished - Aug 2019

Keywords

  • Bacillus subtilis
  • FF-ATPase
  • Ribosome
  • synthases
  • ε subunit

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