Biocompatibility of silver-Containing calcium-Phosphate cements with anti-Bacterial properties

Yusuke Shimizu, Yusuke Kawanobe, Toshiisa Konishi, Nobuyuki Kanzawa, Michiyo Honda, Mamoru Aizawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution


We have previously synthesized silver-containing hydroxyapatite (Ag-HAp) powders by an ultrasonic spray-pyrolysis (USSP) technique. On the other hand, we have successfully fabricated novel calcium-phosphate cements (CPCs) composed of mainly |3-tricalcium phosphate (|3-TCP) phase with anti-washout property (hereafter, |3-TCP cement), which was set on the basis of chelate-bonding ability of inositol phosphate (IP6). In this study, we developed novel CPCs with both anti-bacterial and anti-washout properties by adding the Ag-HAp powder into the above |3-TCP cements, and examined their anti-bacterial property and cytotoxicity. The Ag-HAp powders with Ag contents of 0, 2, and 5 mol% as a nominal composition were synthesized by an USSP technique. The raw powder for β-TCP cement was prepared by ball-milling the commercially-available |3-TCP powder in the IP6 solution. The Ag-HAp/β-TCP powders were prepared by mixing Ag-HAp powder and β-TCP cement powder at a ratio of 25:75 in mass. The Ag-HAp/|3-TCP cement was fabricated by mixing the above-mentioned Ag-HAp/β-TCP powder and 2.5 mass% Na2HP04solution at a powder/liquid ratio of 1/0.3 [g/cm3 ]. The anti-bacterial property of resulting cements was evaluated using Staphylococcus aureus by biofilm formation test. The Ag-HAp/β-TCP cements containing 2 and 5 mol% Ag showed strong anti-bacterial property among examined specimens. Furthermore, the cytotoxicity of Ag+ ion eluted from these cements was also examined using osteoblastic MC3T3-E1 cells and Transwell® kit. The relative cell viability cultured on each Ag-containing cement specimen was over 80 %, compared with the control (polystyrene plate). These results demonstrate that the present Ag-HAp/β-TCP cements containing 2 mol% Ag are promising one of the candidates as CPCs with both anti-bacterial property and biocompatibility.

Original languageEnglish
Title of host publicationBioceramics 26
EditorsMaria-Pau Ginebra, Cristina Canal, Montserrat Espanol, Edgar B. Montufar, Roman A. Perez
PublisherTrans Tech Publications Ltd
Number of pages6
ISBN (Electronic)9783038352822
Publication statusPublished - 1 Jan 2015
Event26th Symposium and Annual Meeting of the International Society for Ceramics in Medicine, ISCM 2014 - Barcelona, Spain
Duration: 6 Nov 20148 Nov 2014

Publication series

NameKey Engineering Materials
ISSN (Print)1013-9826


Conference26th Symposium and Annual Meeting of the International Society for Ceramics in Medicine, ISCM 2014


  • Anti-bacterial property
  • Calcium-phosphate cement
  • Chelate-setting cement
  • Cytotoxicity
  • Inositol phosphate
  • Silver-containing hydroxyapatite

Cite this

Shimizu, Y., Kawanobe, Y., Konishi, T., Kanzawa, N., Honda, M., & Aizawa, M. (2015). Biocompatibility of silver-Containing calcium-Phosphate cements with anti-Bacterial properties. In M-P. Ginebra, C. Canal, M. Espanol, E. B. Montufar, & R. A. Perez (Eds.), Bioceramics 26 (pp. 107-112). (Key Engineering Materials; Vol. 631). Trans Tech Publications Ltd.