Bio-sniffers for ethanol and acetaldehyde using carbon and Ag/AgCl coated electrodes

Tomoko Gessei, Hitoshi Sato, Elito Kazawa, Hiroyuki Kudo, Hirokazu Saito, Kohji Mitsubayashi

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Monitoring of ethanol and acetaldehyde in expired gas after drinking is an effective method for assessment of alcohol metabolic function. We have developed bioelectronic gas sensors (bio-sniffers) for convenient measurement of ethanol and acetaldehyde. The bio-sniffers were fabricated using a simple process and inexpensive method. The process consisted of coating carbon and Ag/AgCl on a filter paper using screen-printing, and immobilizing alcohol oxidase and aldehyde dehydrogenase on the carbon electrode. These bio-sniffers showed good response to ethanol and acetaldehyde vapor and were used to sense these vapors in the concentration range 1.0 to 100 ppm and 0.2 to 4.0 ppm, respectively. The calibration ranges cover the alcohol and acetaldehyde concentration in breath air after drinking. The bio-sniffer for acetaldehyde is applicable also to evaluate other aldehydes (i.e. formaldehyde as volatile organic compounds).

Original languageEnglish
Pages (from-to)179-186
Number of pages8
JournalMicrochimica Acta
Issue number1-2
Publication statusPublished - 1 Apr 2009



  • Acetaldehyde
  • Alcohol metabolic
  • Biosensor
  • Breath analysis
  • Ethanol
  • Gas monitoring

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