Benzophenone synthase from Garcinia mangostana L. pericarps

Natsajee Nualkaew, Hiroyuki Morita, Yoshihiko Shimokawa, Keishi Kinjo, Tetsuo Kushiro, Wanchai De-Eknamkul, Yutaka Ebizuka, Ikuro Abe

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

The cDNA of a benzophenone synthase (BPS), a type III polyketide synthase (PKS), was cloned and the recombinant protein expressed from the fruit pericarps of Garcinia mangostana L., which contains mainly prenylated xanthones. The obtained GmBPS showed an amino acid sequence identity of 77-78% with other plant BPSs belonging to the same family (Clusiaceae). The recombinant enzyme produced 2,4,6-trihydroxybenzophenone as the predominant product with benzoyl CoA as substrate. It also accepted other substrates, such as other plant PKSs, and used 1-3 molecules of malonyl CoA to form various phloroglucinol-type and polyketide lactone-type compounds. Thus, providing GmBPS with various substrates in vivo might redirect the xanthone biosynthetic pathway.

Original languageEnglish
Pages (from-to)60-69
Number of pages10
JournalPhytochemistry
Volume77
DOIs
Publication statusPublished - May 2012

Keywords

  • Benzophenone synthase
  • Clusiaceae
  • Garcinia mangostana
  • Polyketide synthase
  • Xanthones

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