Applications of the spin-trapping method in radiation biology

Mikinori Kuwabara, Wakako Hiraoka, Osamu Inanami

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Ionizing radiation produces many kinds of free radicals in biologically important molecules through direct or indirect actions. In this chapter we have shown that a method that combined ESR, spin trapping and chromatographic separation made it possible to identify free radicals induced by direct ionization as well as OH-radical reactions in nucleic-acid related and protein-related compounds. In addition, combination with enzymatic digestion expanded the potential of this method to detect free radical production in molecules with high molecular weight such as polynucleotides, RNA and DNA, and polypeptides and proteins. The effects of ionizing radiation on biological molecules underlie the primary processes involved in radiation biology. ESR combined with spin trapping remains a useful method for clarifying these processes in a living organism.

Original languageEnglish
Title of host publicationApplications of EPR in Radiation Research
PublisherSpringer International Publishing
Number of pages32
ISBN (Electronic)9783319092164
ISBN (Print)3319092154, 9783319092157
Publication statusPublished - 1 Jun 2014


  • 2-Methyl-2-nitrosopropane
  • DNA, proteins
  • ESR
  • Free radicals
  • HPLC
  • OH radicals
  • Spin trapping
  • X irradiation
  • γ irradiation


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