A rapid analytical method for free choline by LC and its application for bacterial culture medium samples

Masayuki Kuribayashi, Minoru Tsuzuki, Kiichi Sato, Mitsuru Abo, Etsuro Yoshimura

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A rapid, and inexpensive analytical method for free choline is described here. Choline was derivatized with benzoyl chloride and analyzed by HPLC with UV detection. Choline samples in the micro-molar range were determined with good repeatability. The precision (RSD) was not greater than 10%. The quantification limit was 10 μM (3σ), and the linear calibration range was between 10 and 1,500 μM. This method was applied to the measurement of free choline in a culture medium of bacteria and was shown to be suitable for aqueous samples with a wide concentration range and for routine analyses.

Original languageEnglish
Pages (from-to)339-341
Number of pages3
Issue number3-4
Publication statusPublished - 1 Feb 2008


  • Choline
  • Column liquid chromatography
  • Salt stress

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