A novel multifunctional triterpene synthase from Arabidopsis thaliana

Tetsuo Kushiro, Masaaki Shibuya, Kazuo Masuda, Yutaka Ebizuka

Research output: Contribution to journalArticlepeer-review

64 Citations (Scopus)

Abstract

The Arabidopsis thaliana genome sequencing project has identified several triterpene synthase homologues. One cDNA of these clones, YUP8H12R.43, was obtained and functionally expressed in yeast. At least nine triterpenes have been identified as its products based on NMR and LCMS analysis. The products include extensively migrated triterpenes, multiflorenol and bauerenol. Several chimeric clones were constructed between YUP8H12R.43 and A. thaliana lupeol synthase LUP1, to reveal that a C-terminal half and a part of N-terminus is important for such product multiplicity. The presence of such multifunctional triterpene synthase in plants is noteworthy from both a mechanistic and a physiological point of view. (C) 2000 Elsevier Science Ltd.

Original languageEnglish
Pages (from-to)7705-7710
Number of pages6
JournalTetrahedron Letters
Volume41
Issue number40
DOIs
Publication statusPublished - 30 Sep 2000

Keywords

  • Arabidopsis thaliana
  • Oxidosqualene cyclase
  • Triterpene synthase
  • YUP8H12R.43

Fingerprint

Dive into the research topics of 'A novel multifunctional triterpene synthase from Arabidopsis thaliana'. Together they form a unique fingerprint.

Cite this